Candy Electrophoresis Lab
We mixed our Sample yellow and red with the extract so this may not be 100% correct.
Focus Questions
1. When you analyzed the results of your gel, did any of your experimental samples contain dyes that did
not match the four reference dyes? For example, did any of your samples produce:
a. Dye bands that are a different size than any of the reference bands?
b. Dyes that are a different color than any of the reference bands?
c. More than one color band?
d. Dyes that you observed moving in the “wrong” direction (toward the cathode)?
What might these dyes be?
All was similar to the sample dyes except for the brown dye we used. The dyes were not that different though there was some residue that was noticeable in the solution.
2. Look at the structures of the dyes pictured here. Which of these dyes would migrate similarly to the dyes
you examined in this lab?
Why?
Fast green and citrus, these seem the shape that was gonna go as far as our colors that we had.
3. Many popular dry dog foods and dog treats have FD&C dyes among their ingredients. For example,
Beneful dry food contains Yellow 5, Red 40, Yellow 6, and Blue 2, and Snausages Breakfast Bites
contain Red 40 lake, Yellow 6 lake, and Yellow 5 lake. (Lake dyes are the insoluble forms of the FD&C dyes.)
Why do dog food manufacturers put artificial food colors in dog food?
To make the food more appealing to dogs or owners it is colored to make it more view able as food.
4.Are there any reasons why artificial food colors might be preferable to natural food colors?
They are a lot stronger and cheaper then natural dyes.
5. What two factors control the distance the colored dye solutions migrate?
How big the particles were and how much are they negatively charged
6. What force helps move the dyes through the gel?
An electric current
7. What component of the electrophoresis system causes the molecules to separate by size?
Explain
The gel is allowing the bigger particles to move slower due to size and the smaller ones will go faster because they have less to go through.
8. Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
the bigger ones will be stuck and the smaller ones will move forward allowing to separate them and get them at different places.
Focus Questions
1. When you analyzed the results of your gel, did any of your experimental samples contain dyes that did
not match the four reference dyes? For example, did any of your samples produce:
a. Dye bands that are a different size than any of the reference bands?
b. Dyes that are a different color than any of the reference bands?
c. More than one color band?
d. Dyes that you observed moving in the “wrong” direction (toward the cathode)?
What might these dyes be?
All was similar to the sample dyes except for the brown dye we used. The dyes were not that different though there was some residue that was noticeable in the solution.
2. Look at the structures of the dyes pictured here. Which of these dyes would migrate similarly to the dyes
you examined in this lab?
Why?
Fast green and citrus, these seem the shape that was gonna go as far as our colors that we had.
3. Many popular dry dog foods and dog treats have FD&C dyes among their ingredients. For example,
Beneful dry food contains Yellow 5, Red 40, Yellow 6, and Blue 2, and Snausages Breakfast Bites
contain Red 40 lake, Yellow 6 lake, and Yellow 5 lake. (Lake dyes are the insoluble forms of the FD&C dyes.)
Why do dog food manufacturers put artificial food colors in dog food?
To make the food more appealing to dogs or owners it is colored to make it more view able as food.
4.Are there any reasons why artificial food colors might be preferable to natural food colors?
They are a lot stronger and cheaper then natural dyes.
5. What two factors control the distance the colored dye solutions migrate?
How big the particles were and how much are they negatively charged
6. What force helps move the dyes through the gel?
An electric current
7. What component of the electrophoresis system causes the molecules to separate by size?Explain
The gel is allowing the bigger particles to move slower due to size and the smaller ones will go faster because they have less to go through.
8. Agarose electrophoresis is commonly used to separate molecules of DNA. Explain how you expect DNA molecules with molecular weights of 600, 1000, 2000, and 5000 daltons to separate.
the bigger ones will be stuck and the smaller ones will move forward allowing to separate them and get them at different places.
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